Use of Gene Transfer in the Isolation of Cell Surface Receptor Genes

摘要: The study of cell surface receptors is essential for understanding means by which cells communicate with each other. In recent years, there has been a tremendous surge interest in such molecules, large part because several them have shown to be involved regulation growth. particular, both the EGF and CSF-1 oncogenic potential when expressed altered form as viral oncogenes (1,2). Numerous cellular genes encode recently cloned, studies using these are yielding valuable insight into mechanisms activation cell-cell interaction. Most receptor isolated conventional involving protein purification subsequent sequence determination. epidermal growth factor (1), insulin (3), platelet-derived (4), interleukin-2 (5) subjected this approach. Other approaches depended on enrichment mRNA encoding receptor; low density lipoprotein (6) T (7) means. We employed an alternative approach relies DNA mediated gene transfer mammalian detection transfected product (Figure 1).