Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems

摘要: Azotobacter vinelandii, a strict aerobic, nitrogen fixing bacterium in the Pseudomonadaceae family, exhibits preferential use of acetate over glucose as carbon source. In this study, we show that GluP (Avin04150), annotated an H+-coupled glucose-galactose symporter, is transporter A. vinelandii. This protein, which widely distributed bacteria and archaea, uncommon Pseudomonas species. We found expression gluP was under catabolite repression control thorugh CbrA/CbrB Crc/Hfq regulatory systems, were functionally conserved between vinelandii While histidine kinase CbrA essential for utilization, over-expression Crc protein arrested cell growth when sole Hfq proteins from either or P. putida could form stable complex with RNA A-rich Hfq-binding motif present leader region mRNA. Moreover, putida, functional promoted translational inhibition lacZ reporter gene. The fact not genus but systems demonstrates relevance these regulating metabolism family.