The Crc/CrcZ-CrcY global regulatory system helps the integration of gluconeogenic and glycolytic metabolism inPseudomonas putida

摘要: Summary In metabolically versatile bacteria, carbon catabolite repression (CCR) facilitates the preferential assimilation of most efficient sources, improving growth rates and fitness. In Pseudomonas putida, Crc Hfq proteins CrcZ CrcY small RNAs, which are believed to antagonize Crc/Hfq, key players in CCR. Unlike that seen other bacterial species, succinate glucose elicit weak CCR this bacterium. present work, metabolic, transcriptomic constraint-based metabolic flux analyses were combined clarify whether P. putida prefers or glucose, identify role protein metabolism these compounds. When provided simultaneously, was consumed faster than although both compounds metabolized. levels lower when substrates only one provided, suggesting a for coordinating Flux distribution analysis suggested that, present, works organize flow opposite directions: from pyruvate, pyruvate. Thus, our results support not favours preferred compounds, but balances fluxes, optimizing growth.