Recovery of tubulin functions after freeze-drying in the presence of trehalose
作者： Eduarda Dráberová , Vadym Sulimenko , Tetyana Sulimenko , Konrad J Böhm , Pavel Dráber
关键词: Cytoskeleton 、 Cell biology 、 Microtubule 、 Motor protein 、 Trehalose 、 Kinesin 、 Cell morphology 、 Biology 、 Tubulin 、 Microtubule-associated protein 、 Biophysics 、 Biochemistry 、 Molecular biology
摘要: Abstract Microtubules represent cytoplasmic structures that are indispensable for the maintenance of cell morphology and motility generation. Due to their regular structural organization, microtubules have become great interest preparation in vitro nanotransport systems. However, tubulin, major building protein microtubules, is a thermolabile usually stored at −80 °C preserve its conformation polymerization properties. Here we describe novel method freeze-drying assembly-competent tubulin presence nonreducing sugar trehalose. Even after prolonged storage ambient temperature, rehydrated capable binding antimitotic drugs assembling bind microtubule-associated proteins usual way. Electron microscopy confirmed assembles into normal able generate by interaction with motor kinesin cell-free environment. Freeze-drying also preserved preformed microtubules. Rehydrated can be used diverse vivo assays as well bionanodevices.
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