The Role of Kinesin Neck Linker and Neck in Velocity Regulation

摘要: Despite the high level of similarity in structural organisation their motor domains and, consequently, mechanism motility generation, kinesin-5 moves about 25-fold slower than conventional kinesin (kinesin-1). To elucidate motifs contributing to velocity regulation, we expressed a set Eg5- and KIF5A-based chimeric proteins with interchanged native neck linker elements. Among them, chimera consisting Eg5 catalytic core (residues 1-357) fused KIF5A 326-450) displayed increased compared control protein. This is first evidence that slow-moving can be elevated by insertion elements taken from fast-moving motor. Whereas complementary composed (1-325) (358-513) was found immotile, half-KIF5A into this restored motility. Our results indicate are involved not only generation general determination movement direction, but also regulation.