Effects of intraperitoneal hydrogen injection on nitric oxide synthase mRNA and malondialdehyde following limb ischemia-reperfusion in rabbits
作者： Dai-Jun WANG
DOI: 10.3944/AOTT.2015.15.0096
关键词: Nitric oxide synthase 、 Medicine 、 Hindlimb 、 Malondialdehyde 、 Reperfusion injury 、 Intraperitoneal injection 、 Enos 、 Internal medicine 、 Skeletal muscle 、 Surgery 、 Nitric Oxide Synthase Type III 、 Endocrinology 、 Orthopedics and Sports Medicine 、 General Medicine
摘要: Objective: To investigate the effects of intraperitoneal hydrogen (H2) injection on mRNA expression levels inducible nitric oxide synthase (iNOS) and endothelial (eNOS) as well serum malondialdehyde (MDA) level in a rabbit model limb ischemia-reperfusion (I/R)-induced skeletal muscle injury. Methods: establish hind I/R animal model, 30 rabbits were randomly assigned to one three groups: Sham, IRH. An H2 was given IRH group, while an equivalent amount air Sham groups. At 3, 6, 12 24 h post reperfusion, MDA level, iNOS eNOS levels, determined Results: Both higher group than (P < 0.01) lower 0.01 or P 0.05) at various time points after reperfusion. The exhibited no significant difference between groups following reperfusion but significantly 0.05). Conclusions: During process, up-regulated along with increase MDA. Intraperitoneal could down-regulate up-regulate suggesting protective effect I/R-induced
sci-hub.st 参考文章(20)
R M Palmer, S Moncada, E A Higgs, NITRIC OXIDE: PHYSIOLOGY, PATHOPHYSIOLOGY, AND PHARMACOLOGY Pharmacological Reviews. ,vol. 43, pp. 109- 142 ,(1991)
Kentaro Hayashida, Motoaki Sano, Ikuroh Ohsawa, Ken Shinmura, Kayoko Tamaki, Kensuke Kimura, Jin Endo, Takaharu Katayama, Akio Kawamura, Shun Kohsaka, Shinji Makino, Shigeo Ohta, Satoshi Ogawa, Keiichi Fukuda, Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury. Biochemical and Biophysical Research Communications. ,vol. 373, pp. 30- 35 ,(2008) , 10.1016/J.BBRC.2008.05.165
Joseph Nanobashvili, Christoph Neumayer, Alexander Fügl, Andreas Punz, Roland Blumer, Manfred Prager, Martina Mittlböck, Helmut Gruber, Peter Polterauer, Erich Roth, Tadeusz Malinski, Ihor Huk, Ischemia/reperfusion injury of skeletal muscle: plasma taurine as a measure of tissue damage. Surgery. ,vol. 133, pp. 91- 100 ,(2003) , 10.1067/MSY.2003.65
J. Nanobashvili, C. Neumayer, A. Fuegl, E. Sporn, M. Prager, P. Polterauer, T. Malinski1, I. Huk, Ischaemia/Reperfusion Injury of Skeletal Muscle: Mechanisms, Morphology, Treatment Strategies, and Clinical Applications European Surgery-acta Chirurgica Austriaca. ,vol. 34, pp. 83- 89 ,(2002) , 10.1046/J.1563-2563.2002.02029.X
Ikuroh Ohsawa, Kiyomi Nishimaki, Kumi Yamagata, Masahiro Ishikawa, Shigeo Ohta, Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice Biochemical and Biophysical Research Communications. ,vol. 377, pp. 1195- 1198 ,(2008) , 10.1016/J.BBRC.2008.10.156
R. M. J. Palmer, A. G. Ferrige, S. Moncada, Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor Nature. ,vol. 327, pp. 524- 526 ,(1987) , 10.1038/327524A0
Aslı Korkmaz, Dürdane Kolankaya, Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury. Canadian Journal of Surgery. ,vol. 56, pp. 6- 14 ,(2013) , 10.1503/CJS.004811
AYŞEGÜL Küçük, M Yucel, NİLÜFER Erkasap, MURAT Tosun, T Koken, METE Ozkurt, S Erkasap, None, The effects of PDE5 inhibitory drugs on renal ischemia/reperfusion injury in rats Molecular Biology Reports. ,vol. 39, pp. 9775- 9782 ,(2012) , 10.1007/S11033-012-1843-1
Guoqing Huang, Jun Zhou, Wei Zhan, Yan Xiong, Chunlin Hu, Xiangmin Li, Xin Li, Yingqing Li, Xiaoxing Liao, The neuroprotective effects of intraperitoneal injection of hydrogen in rabbits with cardiac arrest Resuscitation. ,vol. 84, pp. 690- 695 ,(2013) , 10.1016/J.RESUSCITATION.2012.10.018
James F. George, Anupam Agarwal, Hydrogen: another gas with therapeutic potential Kidney International. ,vol. 77, pp. 85- 87 ,(2010) , 10.1038/KI.2009.432