Ischemia/reperfusion injury of skeletal muscle: plasma taurine as a measure of tissue damage.

摘要: Background. Cell membrane rupture by oxygen-derived free radicals is a systematic feature of ischemia/reperfusion (I/R) injury. High taurine concentration gradients in skeletal muscle prompted us to evaluate whether plasma levels (pTau) are useful marker I/R injury after different periods ischemia. Methods. Rabbits were randomly assigned either 1 or 2.5 hours hind-limb ischemia followed 2 reperfusion (groups IR1 [n = 12] and IR2.5 13], respectively). Corresponding sham groups (SHAM1 8] SHAM2.5 9]) used as controls. Analyzed parameters included histomorphometry electron microscopy biopsies, pTau, level malondialdehyde. Skeletal function was assessed 3 weeks Results. No significant morphologic changes detectable at the end After reperfusion, mild interstitial edema with intact cell membranes developed group; pTau not increased. group, contrast, showed severe formation (interfiber area increased 112%, P <.005), microvascular constriction (microvessel decreased 33%, <.0005), damage that confirmed higher than group (P <.0005). Pronounced resulted impaired (maximal tetanic tension reduced times, <.005) but group. Conclusion. tolerates h/2 h I/R, latter resulting formation, constriction, late dysfunction. through stimulated lipid peroxidation promotes leakage intracellular taurine, leading may be considered prognostically unfavorable terms organ reversibility. In rabbit model, seems sensitive muscle. (Surgery 2003;133:91-100.)