Rotational orientation of upstream curved DNA affects promoter function in Bacillus subtilis

摘要: Abstract The Alu156 promoter isolated from the Bacillus subtilis bacteriophage SP82 is dependent on curved DNA upstream of -35 region for efficient function. Short insertions 6-29 base pairs were used to simultaneously change linear placement and rotational orientation this relative region. When these mutant promoters analyzed in vivo using transcriptional fusions with a chloramphenicol acetyltransferase gene, changes correlated most contained 11 21 pairs, 15 25 resulted least promoters. importance proper alignment activity was also observed vitro at level transcription RNA polymerase binding. Based electrophoretic mobilities fragments containing various insertion promoters, there second downstream point. findings are consistent idea that deflects helix back toward promoter-bound molecule allow enzyme interact directly DNA. These interactions proposed structure formation open complex.