Selective modulation of NMDA responses by reduction and oxidation.
作者： Elias Aizenman , Stuart A. Lipton , Ralph H. Loring
DOI: 10.1016/0896-6273(89)90310-3
关键词: Glutamate receptor 、 Aspartic acid 、 Dithiothreitol 、 Membrane potential 、 Electrophysiology 、 Biophysics 、 Chemistry 、 Redox 、 Reducing agent 、 Biochemistry 、 NMDA receptor
摘要: Electrophysiological responses to the glutamate analog N-methyl-D-aspartate (NMDA) measured in three different central neuronal preparations are subject a novel modulatory mechanism: they substantially potentiated after exposure disulfide reducing agent dithiothreitol, while oxidation with 5-5-dithiobis-2-nitrobenzoic acid decreases magnitude of response. Modification NMDA response by either or reduction does not appear affect pharmacological properties receptor-channel complex. Since we observe that redox state native complex varies widely among neurons, an vivo mechanism can strongly regulate NMDA-activated functions may exist. In addition, these results suggest it be possible design specific agents for characterizing
elsevier.com
sci-hub.se 参考文章(34)
P N Kao, A J Dwork, R R Kaldany, M L Silver, J Wideman, S Stein, A Karlin, Identification of the alpha subunit half-cystine specifically labeled by an affinity reagent for the acetylcholine receptor binding site. Journal of Biological Chemistry. ,vol. 259, pp. 11662- 11665 ,(1984) , 10.1016/S0021-9258(20)71257-3
PA Rosenberg, Catecholamine toxicity in cerebral cortex in dissociated cell culture. The Journal of Neuroscience. ,vol. 8, pp. 2887- 2894 ,(1988) , 10.1523/JNEUROSCI.08-08-02887.1988
Claus Braestrup, Peter H. Andersen, Effects of Heavy Metal Cations and Other Sulfhydryl Reagents on Brain Dopamine D1 Receptors: Evidence for Involvement of a Thiol Group in the Conformation of the Active Site Journal of Neurochemistry. ,vol. 48, pp. 1667- 1672 ,(1987) , 10.1111/J.1471-4159.1987.TB05721.X
J.M. Tolliver, T.C. Pellmar, Effects of dithiothreitol, a sulfhydryl reducing agent, on CA1 pyramidal cells of the guinea pig hippocampus in vitro. Brain Research. ,vol. 456, pp. 49- 56 ,(1988) , 10.1016/0006-8993(88)90345-9
J. Davies, A.A. Francis, A.W. Jones, J.C. Watkins, 2-Amino-5-phosphonovalerate (2APV), a potent and selective antagonist of amino acid-induced and synaptic excitation Neuroscience Letters. ,vol. 21, pp. 77- 81 ,(1981) , 10.1016/0304-3940(81)90061-6
James M. Tolliver, Terry C. Pellmar, Dithiothreitol elicits epileptiform activity in CA1 of the guinea pig hippocampal slice. Brain Research. ,vol. 404, pp. 133- 141 ,(1987) , 10.1016/0006-8993(87)91364-3
E. H. Wong, J. A. Kemp, T. Priestley, A. R. Knight, G. N. Woodruff, L. L. Iversen, The anticonvulsant MK-801 is a potent N-methyl-D-aspartate antagonist. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 7104- 7108 ,(1986) , 10.1073/PNAS.83.18.7104
L. Nowak, P. Bregestovski, P. Ascher, A. Herbet, A. Prochiantz, Magnesium gates glutamate-activated channels in mouse central neurones Nature. ,vol. 307, pp. 462- 465 ,(1984) , 10.1038/307462A0
D Leifer, S. Lipton, C. Barnstable, R. Masland, Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture Science. ,vol. 224, pp. 303- 306 ,(1984) , 10.1126/SCIENCE.6143400
Arthur Karlin, Eva Bartels, Effects of blocking sulfhydryl groups and of reducing disulfide bonds on the acetylcholine-activated permeability system of the electroplax Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis. ,vol. 126, pp. 525- 535 ,(1966) , 10.1016/0926-6585(66)90010-0