Detection of tet(K) and tet(M) in Staphylococcus aureus of Asian countries by the polymerase chain reaction

摘要: This study describes the use of polymerase chain reaction (PCR) to detect tet(K) and tet(M) tetracycline resistance genes in Staphylococcus aureus. Primers based on DNA sequence from S. aureus were used as primers PCR assay presence for minocycline. Two-hundred fifteen isolates Asian countries Japan, Indonesia, China, Korea Thailand examined, results confirm that specifies but not minocycline both We observed two different types clinical strains resistant tetracycline: first carried only gene, while second genes. Almost all China genes, most aureuss Japan gene.