Studies on the lipolytic activity of sonicated enzymes from Yarrowia lipolytica.
作者： Agata Urszula Kapturowska , Izabela Agnieszka Stolarzewicz , Jolanta Krzyczkowska , Ewa Białecka-Florjańczyk
DOI: 10.1016/J.ULTSONCH.2011.06.015
关键词: Cell disruption 、 Yeast 、 Lipase 、 Denaturation (biochemistry) 、 Sonication 、 Biochemistry 、 Hydrolysis 、 Chromatography 、 Chemistry 、 Yarrowia 、 Solvent
摘要: The aim of this study was to evaluate the efficiency sonication in releasing protein from a widespread lipase-producing yeast, Yarrowia lipolytica KKP 379, and examine impact ultrasound waves generated horn-type sonicator on lipolytic activity Y. hydrolysis p-nitrophenyl laurate. In paper, we focused few parameters cell disruption, such as time sonication, acoustic power, storage frozen yeast biomass used solvent suspend cells which were considered most important part process obtaining biocatalyst for organic synthesis. effective additive release proved be 2% Tween 80; other ideal ultrasonic power at 150 W 15 min 9 weeks time. parameters, best release, did not seem high due denaturation an effect cavitation.
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sci-hub.se 参考文章(25)
L. Ciccolini, P. Taillandier, A.M. Wilhem, H. Delmas, P. Strehaiano, Low frequency thermo-ultrasonication of Saccharomyces cerevisiae suspensions: Effect of temperature and of ultrasonic power Chemical Engineering Journal. ,vol. 65, pp. 145- 149 ,(1997) , 10.1016/S1385-8947(96)03172-5
Rajesh Krishnamurthy, Janice A Lumpkin, Rajagopalan Sridhar, Inactivation of lysozyme by sonication under conditions relevant to microencapsulation. International Journal of Pharmaceutics. ,vol. 205, pp. 23- 34 ,(2000) , 10.1016/S0378-5173(00)00473-7
Ikuko Oyane, Tomo Takeda, Yasunori Oda, Takashi Sakata, Masakazu Furuta, Kenji Okitsu, Yasuaki Maeda, Rokuro Nishimura, Comparison between the effects of ultrasound and γ-rays on the inactivation of Saccharomyces cerevisiae: Analyses of cell membrane permeability and DNA or RNA synthesis by flow cytometry Ultrasonics Sonochemistry. ,vol. 16, pp. 532- 536 ,(2009) , 10.1016/J.ULTSONCH.2009.01.001
Francisco J. Deive, Elisabete Carvalho, Lorenzo Pastrana, Maria L. Rúa, Maria A. Longo, M. Angeles Sanroman, Strategies for improving extracellular lipolytic enzyme production by Thermus thermophilus HB27 Bioresource Technology. ,vol. 100, pp. 3630- 3637 ,(2009) , 10.1016/J.BIORTECH.2009.02.053
A. Lateef, J.K. Oloke, S.G. Prapulla, The effect of ultrasonication on the release of fructosyltransferase from Aureobasidium pullulans CFR 77 Enzyme and Microbial Technology. ,vol. 40, pp. 1067- 1070 ,(2007) , 10.1016/J.ENZMICTEC.2006.08.008
Yoshio Ishimori, Isao Karube, Shuichi Suzuki, Continuous production of glucose oxidase with Aspergillus sp. under ultrasound waves Enzyme and Microbial Technology. ,vol. 4, pp. 85- 88 ,(1982) , 10.1016/0141-0229(82)90086-2
Belma Özbek, Kutlu Ö Ülgen, The stability of enzymes after sonication Process Biochemistry. ,vol. 35, pp. 1037- 1043 ,(2000) , 10.1016/S0032-9592(00)00141-2
Chin Woi Ho, Tsuey Kee Chew, Tau Chuan Ling, Suryani Kamaruddin, Wen Siang Tan, Beng Ti Tey, Efficient mechanical cell disruption of Escherichia coli by an ultrasonicator and recovery of intracellular hepatitis B core antigen Process Biochemistry. ,vol. 41, pp. 1829- 1834 ,(2006) , 10.1016/J.PROCBIO.2006.03.043
Dietrich Knorr, Marco Zenker, Volker Heinz, Dong-Un Lee, Applications and potential of ultrasonics in food processing Trends in Food Science and Technology. ,vol. 15, pp. 261- 266 ,(2004) , 10.1016/J.TIFS.2003.12.001
M.V. Shynkaryk, N.I. Lebovka, J.-L. Lanoisellé, M. Nonus, C. Bedel-Clotour, E. Vorobiev, Electrically-assisted extraction of bio-products using high pressure disruption of yeast cells (Saccharomyces cerevisiae). Journal of Food Engineering. ,vol. 92, pp. 189- 195 ,(2009) , 10.1016/J.JFOODENG.2008.10.041