Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain

摘要: The sensory circumventricular organs (CVOs), which comprise the organum vasculosum of lamina terminalis (OVLT), subfornical organ (SFO) and area postrema (AP), lack a typical blood–brain barrier (BBB) monitor directly blood-derived information to regulate body fluid homeostasis, inflammation, feeding vomiting. Until now, almost nothing has been documented about vascular features CVOs except fenestration endothelial cells. We therefore examine whether continuous angiogenesis occurs in adult mouse. angiogenesis-inducing factor growth factor-A (VEGF-A) VEGF-A-regulating transcription hypoxia-inducible factor-1α were highly expressed neurons OVLT SFO both astrocytes AP. Expression pericyte-regulating platelet-derived B was high CVOs. Immunohistochemistry bromodeoxyuridine Ki-67, nuclear protein that is associated with cellular proliferation, revealed active proliferation Moreover, immunohistochemistry caspase-3 basement membrane marker laminin showed presence apoptosis sprouting cells, respectively. Treatment VEGF receptor-associated tyrosine kinase inhibitor AZD2171 significantly reduced filopodia as well diameter microvessels. mitotic cytosine-b-D-arabinofuranoside cells permeability low-molecular-weight molecules without changing microvessel diameter. Thus, our data indicate dependent on signaling responsible for dynamic plasticity structure permeability.