Investigation on macrokinetics of heterogeneous process of monosaccharide isomerization using non-growing cells of a glucoisomerase producer Arthrobacter nicotianae immobilized inside SiO2-xerogel

摘要: Macrokinetic peculiarites of heterogeneous process monosaccharide (glucose/fructose) isomerization using biocatalysts prepared by incorporation non-growing cells a glucose isomerase-producing strain Arthobacter nicotianae inside SiO2-xerogel have been investigated. It was shown that the proceeds in kinetic regime without diffusion limitation and biocatalyst activities at 60 75°C were 19 32 U/g, respectively. Time equilibrium reaction function starting (“triggering”) isomerase activity unit volume. When exceeds 10 U/ml, equimolar mixture fructose produced within few hours. established continuous carried out plug-flow packed-bed reactor is more efficient than batch accompanied with recycling, first all, to significant improvement operation stability designed biocatalysts. Under model conditions industrial producing glucose-fructose syrups, half-life time inactivation 500 h (65 ± 5)°C.