Isolation and characterisation of intact steryl ferulates from seeds

摘要: Abstract Approaches to the isolation and characterisation of intact steryl ferulates were investigated. Alkaline washing total lipid extract obtained from seeds allowed preliminary separation a phenolic ester containing fraction abundant neutral lipids (comprising largely triacylglycerides, sterols fatty acyl esters). Subsequent thin-layer chromatography (silica gel) afforded purified ferulates. Separation individual molecular species was achieved by reversed-phase (ODS) high-performace liquid (HPLC) using acetate derivatives ferulates, or high-temperature gas (GC) underivatised their acetates trimethylsilyl ethers. Characterisation based on electron impact mass spectra GC—mass spectrometry (MS) ethers, direct insertion probe MS preparative HPLC. The feasibility above approach established well characterised ferulate mixture γ-oryzanol rice bran oil. methods subsequently applied analysis present in maize ( Zea mays ).