Induction of Neutrophil Chemotaxis by the Quorum-Sensing Molecule N-(3-Oxododecanoyl)-l-Homoserine Lactone

摘要: Acyl homoserine lactones are synthesized by Pseudomonas aeruginosa as signaling molecules which control production of virulence factors and biofilm formation in a paracrine manner. We found that N-(3-oxododecanoyl)-L-homoserine lactone (3OC12-HSL), but not its 3-deoxo isomer or acyl-homoserine with shorter fatty acids, induced the directed migration (chemotaxis) human polymorphonuclear neutrophils (PMN) vitro. By use selective inhibitors pathway, comprising phosphotyrosine kinases, phospholipase C, protein kinase mitogen-activated could be delineated. In contrast to well-studied chemokines complement C5a interleukin 8, chemotaxis did depend on pertussis toxin-sensitive G proteins, indicating 3OC12-HSL uses another pathway. Strong evidence for presence receptor PMN was derived from uptake studies; radiolabeled 3OC12-HSL, specific saturable binding seen. Taken together, our data provide recognize migrate toward source (that is, site developing biofilm). propose this early attraction contribute prevention formation.