Comparison of extraction solvents and techniques used for the assay of isoflavones from soybean

摘要: The impact of extraction solvents and techniques on the assay isoflavones from soybean was investigated. This systematic study undertaken to address substantial variations in procedures used for soybeans by different research groups as described recent peer-reviewed published literature. Comparison four previously optimized commonly deployed solvent mixtures (acetonitrile:water 58:42 (v/v); ethanol:water 70:30 methanol:water 90:10 superheated pressurized water) carried out isoflavones. In addition, we also examined efficiencies three additional new (dimethyl sulphoxide:acetonitrile:water, 5:58:37 (v/v/v); dimethyl sulphoxide:ethanol:water, 5:70:25 Genapol:water 5:95 (v/v)) soybeans. Assessments six (shaking, vortexing, sonication, stirring, Soxhlet, liquid (PLE)) with an mixture performed. Both, total content HPLC profile varied significantly techniques. Optimum recoveries samples were obtained sulphoxide:ethanol:water (5:75:25, v/v/v) using a PLE. Intermediate other tested. shaker, vortex, Soxhlet between 65% 70% compared Total extracted sonication procedure 93.3%