Immunological detection and localization of the cotton endophyte Enterobacter asburiae JM22 in different plant species

摘要: Immunological methods were used to study the colonization of internal tissues different plant species by endophytic bacterium Enterobacter asburiae JM22. Polyclonal and monoclonal antibodies applied in enzyme-linked immunosorbent assay (ELISA), dot blot assay, tissue printing, or immunogold labeling sensitive specific enough detect JM22 tissues. Detection limits 1.0 x 103 colony-forming units (CFUs)/mL for lo4 CFUs/mL ELISA lo5 assay. showed a positive immunological reaction with nearly all tested spp. In contrast polyclonal antibodies, differentiated closely related genera like Pantoea Serratia. Other bacterial genera, sap from nontreated field-grown crops, soil solutions did not react antisera. When as seed treatment, colonized roots, stems, cotyledons bean, cucumber, cotton plants. Fourteen days after inoculation leaves, was detected inside inoculated bacteria moved roots. reached concentrations up CFUs/g lo3 leaves. Population densities varied between species, being highest bean lowest cotton. growth media. While sand, ground clay, loamy sand high comparable readings, extinctions sandy loam Promix significantly lower than ones other three media, indicating strong influence mixes on reactions. an intensive gold label drop preparations suspensions phosphate buffer, sap, ultrathin sections tissue. After located root surface, concentrated grooves epidermal cells, below collapsed within intercellular spaces cortex close conducting elements. Inoculation leaves resulted occurrence many labeled cells petiole surfaces. colonizes establishes populations various