Isolation, in planta detection, and uses of endophytic bacteria for plant protection.

摘要: This chapter reviews endophytic bacteria and outlines procedures useful in establishing their presence, culture, physiological metabolic interactions with the host biotechnological uses. Alternatively, full-strength bleach may be used as described for fungal isolation. Isolation techniques modified to include isolating xylem-inhabiting from woody stems, although such make poor endophytes The roots are plated on one or more media bacterial isolations. It necessary cut off discard end of each root when it is removed chloramine-T solution. Two general methods isolate surface-disinfected plant material: tissue direct physical extraction isolation techniques. McInroy Kloepper recommend use three a routine screening procedure during bacteria: medium R2A (Difco) oligotrophic bacteria, TSA culturable heterotrophic SC growth fastidious organisms. major has been control diseases, manner which this accomplished subject current research. uses phytoremediation range reducing petroleum hydrocarbon contamination soils soil heavy-metal concentrations, including water-soluble volatile organic xenobiotics, benzene, toluene, ethylbenzene, high-ammonia waste animal manure, chloroform, dichloromethane, xylene, other hydrophobic pollutants.