Monoclonal antibodies to rat plasma kininogen.
作者： Gurrinder S. Bedi , Nathan Back
DOI: 10.1007/978-1-4684-5143-6_18
关键词: Monoclonal antibody 、 Cell culture 、 Antibody 、 Molecular biology 、 Chemistry 、 Spleen 、 Isotype 、 Kininogen 、 Epitope 、 Gel electrophoresis
摘要: Spleen cells from Balb/c mice immunized with purified rat plasma kininogen were fused to P-3 mouse myeloma cells. Positive clones identified by enzyme linked immunosorbent assay (ELISA), cloned successively two times limiting dilution and expanded as ascites tumors. Five hybridomas developed that produced monoclonal antibodies against kininogen. Two of the secreted IgG1(k) isotype remaining three IgG1(λ), IgG2A(k) IgM(k) isotypes respectively. The specificity was confirmed immunoprecipitation coupled Sepharose-4B followed SDS-polyacrylamide gel electrophoresis. These recognize at least distinct epitopes on
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