A new vasopeptide formed by the action of a Murphy-Sturm lymphosarcoma acid protease on rat plasma kininogen.
作者： Gurrinder S. Bedi , Joseph Balwierczak , Nathan Back
DOI: 10.1016/0006-291X(83)91509-7
关键词:
摘要: A new vasoactive peptide, formed by the action of a Murphy-Sturm lymphosarcoma acid protease on rat plasma kininogen was purified gel filtration Sephadex G-50 (fine) and fractions assayed isolated uterus for smooth muscle stimulating activity. The most active fraction further CM-cellulose chromatography. High voltage electrophoresis showed peptide to be one component (Mgly 2.49) with an electrophoretic mobility different from bradykinin, lysyl-bradykinin methionyl-lysyl-bradykinin. molecular weight estimated G-25 column 1460. amino composition determined carboxyl terminal sequence identified carboxypeptidase Y treatment Pro-Phe-Arg-Leu. Dansyl-Edman procedure yielded Ile-Ser-Arg-Pro. produced dose-dependent contraction guinea pig anterior mesenteric vein relaxed rabbit superior artery contracted phenylephrine.
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sci-hub.se 参考文章(11)
Alan J. Barrett, Proteinases in Mammalian Cells and Tissues ,(1977)
R.E. Stephens, Fluorescent thin-layer peptide mapping for protein identification and comparison in the subnanomole range Analytical Biochemistry. ,vol. 84, pp. 116- 126 ,(1978) , 10.1016/0003-2697(78)90490-6
D. H. Spackman, W. H. Stein, Stanford. Moore, Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry. ,vol. 17, pp. 1107- 1115 ,(1958) , 10.1021/AC60139A006
Hans J. Wilkens, Nathan Back, Reversal of bradykinin action: possible involvement of prostaglandins or dual receptors. Pharmacological Research Communications. ,vol. 12, pp. 411- 422 ,(1980) , 10.1016/S0031-6989(80)80111-1
Kenneth R. Woods, Kung-Tsung Wang, Separation of dansyl-amino acids by polyamide layer chromatography Biochimica et Biophysica Acta. ,vol. 133, pp. 369- 370 ,(1967) , 10.1016/0005-2795(67)90078-5
Caroline W. Easley, Ben J.M. Zegers, Marleen De Vijlder, Application of specialized techniques for specific staining of peptide maps on various media Biochimica et Biophysica Acta. ,vol. 175, pp. 211- 213 ,(1969) , 10.1016/0005-2795(69)90161-5
Murray R. Summers, Gary W. Smythers, Stephen Oroszlan, Thin-layer chromatography of sub-nanomole amounts of phenylthiohydantoin (PTH) amino acids on polyamide sheets Analytical Biochemistry. ,vol. 53, pp. 624- 628 ,(1973) , 10.1016/0003-2697(73)90114-0
Lowell M. Greenbaum, Gloria Semente, Ajai Prakash, Steven Roffman, Leukokinins; their pharmacological properties and role in the pathology of fluid (ascites) accumulation Agents and Actions. ,vol. 8, pp. 80- 84 ,(1978) , 10.1007/BF01972406
Marina L. Reis, Liyoko Okino, M.Rocha e Silva, Comparative pharmacological actions of bradykinin and related kinins of larger molecular weights Biochemical Pharmacology. ,vol. 20, pp. 2935- 2946 ,(1971) , 10.1016/0006-2952(71)90096-7
D Regoli, J Barabé, Pharmacology of bradykinin and related kinins. Pharmacological Reviews. ,vol. 32, pp. 1- 46 ,(1980)