Rapid identification of Escherichia coli and Helicobacter pylori in biological samples by capillary zone electrophoresis
作者： Ewa Kłodzińska , Hanna Dahm , Henryk Róźycki , Jacek Szeliga , Marek Jackowski
关键词: Enterobacteriaceae 、 Stomach 、 Helicobacter pylori 、 Escherichia coli 、 Microbiology 、 Chemistry 、 Bacteria 、 Electrophoresis 、 Capillary electrophoresis 、 Microorganism
摘要: The differences in surface charge of different bacteria can be exploited for their separation by capillary electrophoresis. However, this method microorganisms is beset with various drawbacks such as adhesion to the fused silica or cluster formation. To overcome these phenomena we investigated addition poly(ethylene oxide) a focusing agent running buffer and used calcium myoinositol hexakisphosphate specific ions that interact bacterial surface, changing its electrical properties electrophoretic mobilities. In present work, applied CZE identification E. coli infected urine (direct injection) from patients urinary tract infections Helicobacter pylori, which gram-negative bacillus responsible one most common found humans worldwide. pylori colonize stomach are severe diseases gastric tract, ranging chronic ulcer cancer.
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