PCR-Denaturing Gradient Gel Electrophoresis and Two Feces Antigen Tests for Detection of Helicobacter pylori in Mice

摘要: PCR-denaturing Gradient Gel Electrophoresis (PCR-DGGE), a method suitable for the detection of microbial species in complex ecosystems, was evaluated and identification Helicobacter spp. feces stomach tissue mice. Two commercially available stool antigen tests clinical diagnostics humans were also C57B1/6 mouse model H. pylori infection. PCR-DGGE detected only ganmani from pylori-infected as well control animals, whereas specimens it demonstrated presence challenged animals. Hence, DNA predominant spp., which shown cell dilution experiments. The Amplified IDEIA Hp StAR test all infected animals generated no false-positive results, Premier Platinum HpSA-test but or equivocal results 50% HpSA, opposed to StAR, cross-reacted with non-pylori vitro.