Comparative evaluation of planktonic and biofilm modes of growth in salmonella typhimurium

摘要: In this research, comparative studies of planktonic and biofilm modes growth in Salmonella enterica serovar Typhimurium were undertaken. Chemical disinfectant performed for both biofilms at different ages. A gas chromatography coupled to mass spectrometry (GC-MS) metabolite profiling approach was used evaluate metabolic variations between Biofilm curves generated assess their ability grow as a the minimum eradication concentration (MBEC) assay system, achieve most stable carry out susceptibility testing. Growth demonstrated that grown nutrient poor conditions produced over seven-day period such, Results showed more resistant disinfectants compared Salmonella, but increasing age did not increase resistance disinfectants. By using GC-MS metabolomics approach, media with supernatants from cultures (extracellular metabolites); cell extracts those cells (intracellular metabolites). Principal component analysis (PCA) spectral comparisons revealed differences overall profiles S. Typhimurium. The ages subsequently obtained evaluated. PCA no significant intracellular On other hand, extracellular supernatant. In conclusion, increased older biofilms. Metabolic shows there exist chemical Salmonella. Finally, ages, however such observed