Altered expression of microRNAs in the neuronal differentiation of human Wharton’s Jelly mesenchymal stem cells
作者： Hong Zhuang , Rong Zhang , Shujie Zhang , Qinmeng Shu , Dan Zhang
DOI: 10.1016/J.NEULET.2015.05.061
关键词: microRNA 、 Microarray analysis techniques 、 Wharton's jelly 、 Biology 、 Nestin 、 Mesenchymal stem cell 、 Umbilical cord 、 Molecular biology 、 Differentially expressed mirnas 、 Neuronal differentiation
摘要: Abstract Mesenchymal stem cells (MSCs) have the capacity to generate multiple tissues of mesodermal origin, and also potential trans-differentiate into neurons. We isolated MSCs from Wharton’s jelly human umbilical cord (WJ-MSCs), efficiently induced WJ-MSCs neuron-like using a modified method. After neuronal induction for 12 days, most expressed mature marker MAP2 (83 ± 7%), meanwhile some adopted morphology. Nestin (34 ± 6%), NSE (30 ± 5%), GFAP (12 ± 3%). Moreover, we used miRNA microarray analyze differentially miRNAs in differentiation WJ-MSCs. Microarray analysis revealed discrepant profiles uninduced derived Six were chosen further qRT-PCR validation. Among these 6 miRNAs, four (miR-1290, miR-26b, miR-194, miR-124a) up-regulated 2 (miR-4521 miR-543) down-regulated In conclusion, could be cells. More importantly, our findings suggested that might play important roles
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sci-hub.se 参考文章(25)
Erika Varkonyi-Gasic, Rongmei Wu, Marion Wood, Eric F Walton, Roger P Hellens, Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs Plant Methods. ,vol. 3, pp. 12- 12 ,(2007) , 10.1186/1746-4811-3-12
Byoung J. Kim, Jeong H. Seo, James K. Bubien, Young S. Oh, Differentiation of adult bone marrow stem cells into neuroprogenitor cells in vitro. Neuroreport. ,vol. 13, pp. 1185- 1188 ,(2002) , 10.1097/00001756-200207020-00023
H. Dill, B. Linder, A. Fehr, U. Fischer, Intronic miR-26b controls neuronal differentiation by repressing its host transcript, ctdsp2 Genes & Development. ,vol. 26, pp. 25- 30 ,(2012) , 10.1101/GAD.177774.111
J. Sanchez-Ramos, S. Song, F. Cardozo-Pelaez, C. Hazzi, T. Stedeford, A. Willing, T.B. Freeman, S. Saporta, W. Janssen, N. Patel, D.R. Cooper, P.R. Sanberg, Adult bone marrow stromal cells differentiate into neural cells in vitro. Experimental Neurology. ,vol. 164, pp. 247- 256 ,(2000) , 10.1006/EXNR.2000.7389
Louise Alessandra Mesentier-Louro, Camila Zaverucha-do-Valle, Almir Jordao da Silva-Junior, Gabriel Nascimento-dos-Santos, Fernanda Gubert, Ana Beatriz Padilha de Figueirêdo, Ana Luiza Torres, Bruno D Paredes, Camila Teixeira, Fernanda Tovar-Moll, Rosalia Mendez-Otero, Marcelo F Santiago, None, Distribution of Mesenchymal Stem Cells and Effects on Neuronal Survival and Axon Regeneration after Optic Nerve Crush and Cell Therapy PLoS ONE. ,vol. 9, pp. e110722- ,(2014) , 10.1371/JOURNAL.PONE.0110722
Zhao-Jun Liu, Ying Zhuge, Omaida C. Velazquez, Trafficking and differentiation of mesenchymal stem cells Journal of Cellular Biochemistry. ,vol. 106, pp. 984- 991 ,(2009) , 10.1002/JCB.22091
Shao-Fang Zhu, Zhi-Nian Zhong, Xia-Fei Fu, Dong-Xian Peng, Guo-Hui Lu, Wen-Hu Li, Hong-Yan Xu, Hong-Bo Hu, Jian-Ming He, Wei-Yan Su, Yuan-Li He, Comparison of cell proliferation, apoptosis, cellular morphology and ultrastructure between human umbilical cord and placenta-derived mesenchymal stem cells. Neuroscience Letters. ,vol. 541, pp. 77- 82 ,(2013) , 10.1016/J.NEULET.2013.03.018
Mette Christensen, Gerhard M. Schratt, microRNA involvement in developmental and functional aspects of the nervous system and in neurological diseases Neuroscience Letters. ,vol. 466, pp. 55- 62 ,(2009) , 10.1016/J.NEULET.2009.04.043
Dale Woodbury, Emily J. Schwarz, Darwin J. Prockop, Ira B. Black, Adult rat and human bone marrow stromal cells differentiate into neurons Journal of Neuroscience Research. ,vol. 61, pp. 364- 370 ,(2000) , 10.1002/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C
Donald G. Phinney, Darwin J. Prockop, Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair--current views. Stem Cells. ,vol. 25, pp. 2896- 2902 ,(2007) , 10.1634/STEMCELLS.2007-0637