NUCLEAR GENE DOSAGE EFFECTS ON MITOCHONDRIAL MASS AND DNA
作者： Gary W. Grimes , Henry R. Mahler , Philip S. Perlman
DOI: 10.1083/JCB.61.3.565
关键词: Gene dosage 、 Genome 、 Ploidy 、 Mitochondrial DNA 、 Nuclear gene 、 Molecular biology 、 Biology 、 Saccharomyces cerevisiae 、 Mating of yeast 、 Mitochondrion
摘要: In order to assess the effect of nuclear gene dosage on regulation mitochondria we have studied serial sections a set isogenic haploid and diploid cells Saccharomyces cerevisiae, growing exponentially in absence catabolite repression, determined amount mitochondrial DNA per cell. Mitochondria accounted for 14% cytoplasmic 12% total cellular volume all examined regardless their ploidy or apparent stage cell cycle. The mean number was 22 10 haploids. distribution appeared unimodal identical haploids diploids. 12.6 ± 1.2% 13.5 1.3% populations, respectively. These values correspond 3.6 x 10-15 g, 2.2 109 daltons, 44 genomes (50 106 daltons each) twice that On this basis, average mitochondrion these contains four both diploid.
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