Improved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase gene
作者： Ryutaro Aida , Takako Narumi , Norihiro Ohtsubo , Hiroyasu Yamaguchi , Ko Kato
DOI: 10.5511/PLANTBIOTECHNOLOGY.25.69
关键词: Untranslated region 、 Biology 、 Torenia 、 Genetics 、 Molecular biology 、 Translational efficiency 、 RNA 、 Gene 、 Cauliflower mosaic virus 、 Chrysanthemum morifolium 、 Torenia fournieri
摘要: We investigated the efficiency of 5� -untranslated region (UTR) tobacco alcohol dehydrogenase gene (NtADH-5� UTR) as a translational enhancer in chrysanthemum (Chrysanthemum morifolium) and torenia (Torenia fournieri). Three constructs were introduced: control, "ADH� ," cauliflower mosaic virus 35S RNA (CaMV 35S) promoter::b-glucuronidase (GUS); NtADH-5� UTR with spacer, "ADHS," CaMV promoter::NtADH-5� UTR::25-bp spacer::GUS; no UTR::GUS. The highest GUS activity ADHS ADHfor was about 45 190 times, respectively, for 12 22 than that ADHplants. enhanced both species. With lowest set to 1, relative efficiencies 1 15 (ADH� ), 47 568 (ADHS), 267 1360 ) chrysanthemum, 3 114 85 226 torenia. would facilitate practical breeding fundamental genetic research
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