Changes in both gp120 and gp41 can account for increased growth potential and expanded host range of human immunodeficiency virus type 1.

摘要: Virus derived from an infectious molecular clone of the ELI strain human immunodeficiency virus type 1 (HIV-1) replicates well in peripheral blood mononuclear cells and some CD4-positive cell lines but exhibits a delayed time course infection CEM H9 fails to infect SupT1 U937 cells. If that emerges infected is used cells, accelerated able In this study, we technique polymerase chain reaction-single-strand conformation polymorphism localize changes both extracellular gp120 transmembrane gp41 components envelope gene associated with adaptation growth tissue culture lines. Specifically, mutations were identified region implicated CD4 binding amino-terminal portion adjacent involved fusion. No found V3 loop gp120, previously shown be viral tropism. When these introduced into original clone, they conferred enhanced replicative capacity on ELI. These results demonstrate two additional determinants HIV-1 protein influence tropism vitro. They also may have important implications for generation viruses increased potential expanded host range seen late stages HIV disease.