Human immunodeficiency virus type 1 evolution in vivo tracked by DNA heteroduplex mobility assays.

摘要: High mutation rates and strong selective pressures imposed on human immunodeficiency viruses in vivo result the formation of pools genetic variants known as quasispecies. DNA heteroduplex mobility tracking analyses were used to monitor generation HIV sequence diversity, estimate quasispecies complexity, assess turnover approach an understanding relationship between viral evolution disease progression. Proviral nearly homogeneous soon after sexual transmission. The emergence clearance individual then occurred at different individuals. complexity was found long-term-infected, asymptomatic individuals, while rapid CD4+ cell decline AIDS often, but not always, associated with lower complexity. variation often low following vitro culture, because outgrowth one or a few that became more abundant only later proviruses peripheral blood mononuclear cells. These studies provide insight into dynamics virus changes illustrate utility analysis for study phenomena changes.