Endometrial IL-1β, IL-6 and TNF-α, mRNA expression in mares resistant or susceptible to post-breeding endometritis: Effects of estrous cycle, artificial insemination and immunomodulation

摘要: Abstract Endometrial mRNA expression of the pro-inflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) was assessed in mares resistant (RM) or susceptible (SM) to persistent post-breeding endometritis (PPBE). Eight RM eight SM, were selected based on reproductive records functional tests out a herd 2000 light cross-type mares. Three experiments done study transcription patterns (i) basal conditions; (ii) after artificial insemination (AI); (iii) administration an immunomodulator at time insemination. biopsies taken during consecutive cycles: estrus, when follicles reached 35 mm diestrus (7±1 days ovulation); 24 h post-AI, with dead semen (estrus) diestrus; treatment Mycobacterium phlei cell-wall extract (MCWE) preparation AI (with semen), diestrus. quantitated by real PCR. Under conditions, SM had significantly higher all estrus IL-1β TNF-α diestrus, compared RM. After AI, there no differences between estrus; however, for three than under conditions. In showed lower SM. When MCWE administered cytokine induction from found. Globally, correlated well among themselves high. The present that conditions effect estrous cycle; upregulated both persistance latter; down-regulated IL-1 significant effects which behaved like Immunomodulation could be help restoring homeostatic local inflammatory mechanisms, thus assisting prophylaxis