Creation of mouse embryonic stem cell-derived cardiac cell sheets.

摘要: Research on heart tissue engineering is an exciting and promising area. Although we previously developed bioengineered myocardium using cell sheet-based technologies, the issue of appropriate sources remained unresolved. In present study, created sheets mouse embryonic stem (ES) cell-derived cardiomyocytes after expansion in three-dimensional stirred suspension cultures. Serial treatment cultures with noggin granulocyte colony-stimulating factor significantly increased number by more than fourfold compared untreated After drug selection for ES cells expressing neomycin-resistance gene under control α-myosin heavy chain promoter, almost all showed spontaneous beating expressed several cardiac contractive proteins a fine striated pattern. When ES-derived alone were seeded onto temperature-responsive culture dishes, not created, whereas cocultures fibroblasts promoted sheet formation. The beat spontaneously synchronously, connexin 43 at edge adjacent cardiomyocytes. Furthermore, when extracellular action potential was recorded, unidirectional propagation observed. findings suggest that growth factors are capable producing effectively easily, may be tool development myocardium.