Arthritis imaging using a near-infrared fluorescence folate-targeted probe

摘要: A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and KRN transgenic mice serum induction mouse model. In the model, fluorescence signal intensity NIR2-folate (n = 12) free NIR2 5) compared between lipopolysaccharide-treated control joints. The at inflammatory joints found to be significantly higher than normal (up 2.3-fold, P < 0.001). NIR2-free dye group showed persistent lower enhancement ratio group. Excessive folic acid also given demonstrate competitive effect with NIR2-folate. transfer 4), applied different time points after transfer, inflamed could detected as early 30 hours arthritogenic antibody (1.8-fold increase intensity). Fluorescence microscopy, histology, immunohistochemistry validated optical results. We conclude that detection feasible folate-targeted probe. This receptor-targeted method may facilitate improved diagnosis assessment disease progress by providing an characterization active macrophage status joint diseases.