A new enzyme-linked lectin/mucin antibody sandwich assay (CAM 17.1/ WGA) assessed in combination with CA 19-9 and peanut lectin binding assay for the diagnosis of pancreatic cancer
作者： N. Parker , C. A. Makin , C. K. Ching , D. Eccleston , O. M. Taylor
DOI: 10.1002/1097-0142(19920901)70:5<1062::AID-CNCR2820700509>3.0.CO;2-P
关键词: Lectin 、 Pancreatic disease 、 Wheat germ agglutinin 、 Pancreatic cancer 、 Molecular biology 、 CA19-9 、 Medicine 、 Radioimmunoassay 、 Peanut agglutinin 、 Immunology 、 Ligand binding assay 、 Cancer research 、 Oncology
摘要: Background. Mucus glycoproteins are often present in the sera of patients with pancreatic cancer, and their detection quantification can be used serologic diagnosis. Methods. A novel enzyme-linked “sandwich” assay (CAM 17.1/WGA) has been developed which a lectin, wheat germ agglutinin (WGA), is bound to solid phase capture serum glycoproteins, after addition test sera, monoclonal antimucin antibody 17.1) peroxidase-tagged second as system. Results. The applied from 79 cancer 120 controls. CAM 17.1/WGA alone had sensitivity 78% specificity 76% diagnosis cancer. Combination previously described peanut lectin binding (PNA/ELLA) provided 92% 70%, whereas combination CA 19-9 radioimmunoassay 85% 76%. all three tests 94% 66%. In nonjaundiced patients, PNA/ELLA 93% 79% cancer. Conclusions. This new adds significantly armamentarium for These particularly effective when detect different mucin-borne carbohydrate antigens. They deserve more widespread use, examining unexplained abdominal pain or weight loss.
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