Cigarette smoke suppresses type I interferon-mediated antiviral immunity in lung fibroblast and epithelial cells.
作者： Carla MT Bauer , Stephanie J DeWitte-Orr , Kyle R Hornby , Caleb CJ Zavitz , Brian D Lichty
关键词: Interferon 、 ISG15 、 Cancer research 、 Fibroblast 、 Immunology 、 STAT1 、 Interferon type I 、 Biology 、 Transcription factor 、 Cell culture 、 Interferon inducer
摘要: The objective of this study was to investigate the impact cigarette smoke on innate antiviral defense mechanisms; specifically, we examined effects induction type I interferon (IFN). We observed a dose-dependent decrease in ability human lung fibroblast and epithelial cells elicit an response against viral double-strand RNA (dsRNA) mimic, polyI:C, presence smoke-conditioned medium (SCM). Mechanistically, SCM decreases expression IFN-stimulated gene 15 (ISG15) IFN regulatory factor-7 (IRF-7) transcripts suppresses nuclear translocation key transcription factors, factor-kappaB (NF-kappaB) IRF-3, after polyI:C stimulation. Furthermore, provide evidence that intercellular strategy infection is also impaired. fibroblasts state IFN-beta This associated with decreased phosphorylated Stat1 treatment. elicited by are reversible almost entirely abrogated antioxidant, such as glutathione. Our findings suggest affects immediate-early, inductive, amplification phases response.
wlu.ca
core.ac.uk
liebertonline.com参考文章(49)
Russell W. Hudgens, Gerald Sonnenfeld, Effect of sidestream and mainstream smoke exposure on in vitro interferon-alpha/beta production by L-929 cells. Cancer Research. ,vol. 46, pp. 2779- 2783 ,(1986)
Martti Laan, Steven Bozinovski, Gary P. Anderson, Cigarette Smoke Inhibits Lipopolysaccharide-Induced Production of Inflammatory Cytokines by Suppressing the Activation of Activator Protein-1 in Bronchial Epithelial Cells Journal of Immunology. ,vol. 173, pp. 4164- 4170 ,(2004) , 10.4049/JIMMUNOL.173.6.4164
Shin-ichi Tamura, Takeshi Kurata, Defense mechanisms against influenza virus infection in the respiratory tract mucosa. Japanese Journal of Infectious Diseases. ,vol. 57, pp. 236- 247 ,(2004)
C Daly, N C Reich, Double-stranded RNA activates novel factors that bind to the interferon-stimulated response element. Molecular and Cellular Biology. ,vol. 13, pp. 3756- 3764 ,(1993) , 10.1128/MCB.13.6.3756
David Bernhard, Christian W. Huck, Thomas Jakschitz, Gerald Pfister, Blair Henderson, Günther K. Bonn, Georg Wick, Development and evaluation of an in vitro model for the analysis of cigarette smoke effects on cultured cells and tissues. Journal of Pharmacological and Toxicological Methods. ,vol. 50, pp. 45- 51 ,(2004) , 10.1016/J.VASCN.2004.01.003
Irfan Rahman, Saibal K Biswas, Aruna Kode, Oxidant and antioxidant balance in the airways and airway diseases European Journal of Pharmacology. ,vol. 533, pp. 222- 239 ,(2006) , 10.1016/J.EJPHAR.2005.12.087
Ling-Yi L. Chang, Meera Subramaniam, Bradley A. Yoder, Brian J. Day, Misoo C. Ellison, Mary E. Sunday, James D. Crapo, A catalytic antioxidant attenuates alveolar structural remodeling in bronchopulmonary dysplasia. American Journal of Respiratory and Critical Care Medicine. ,vol. 167, pp. 57- 64 ,(2003) , 10.1164/RCCM.200203-232OC
Edgar Schreiber, Keith Harshman, Iris Kemler, Ursula Malipiero, Walter Schaffner, Adriano Fontana, Astrocytes and glioblastoma cells express novel octamer-DNA binding proteins distinct from the ubiquitous Oct-1 and B cell type Oct-2 proteins Nucleic Acids Research. ,vol. 18, pp. 5495- 5503 ,(1990) , 10.1093/NAR/18.18.5495
Y.- T. Juang, W. Lowther, M. Kellum, W.- C. Au, R. Lin, J. Hiscott, P. M. Pitha, Primary activation of interferon A and interferon B gene transcription by interferon regulatory factor 3. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 9837- 9842 ,(1998) , 10.1073/PNAS.95.17.9837
C JEFFERIES, K FITZGERALD, Interferon gene regulation: not all roads lead to Tolls Trends in Molecular Medicine. ,vol. 11, pp. 403- 411 ,(2005) , 10.1016/J.MOLMED.2005.07.006