Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples.

摘要: The purpose of this study was threefold: to compare semen and first void urine (FVU) specimens from asymptomatic infertile men for the detection Chlamydia trachomatis, genital ureaplasma, mycoplasma infections using in-house inhibitor-controlled polymerase chain reaction (PCR)-microtiter plate hybridization assay; determine prevalence those organisms in Tunisia; relationship between these bacteria male infertility. Paired 104 patients were examined by PCR presence DNA ureaplasmas (Ureaplasma urealyticum Ureaplasma parvum) mycoplasmas (Mycoplasma hominis Mycoplasma genitalium). Semen analysis assessed according guidelines World Health Organization. Nominal scale variables, Mann-Whitney test, Kruskal-Wallis nonparametric variance test used statistical analysis. There a very high concordance (>95%) good agreement (kappa > 0.9) ureaplasmas, corresponding FVU specimens. Our findings also show (81.1%) = 0.79) genitalium both C mycoplasmas, found be widespread among Tunisia, as shown their respective prevalences 43.3%, 18.3%, 14.4%. mean values seminal volume, sperm concentration, viability, motility, morphology, leukocyte count not significantly related either trachomatis or that ureaplasma Using our PCR, sensitive diagnostic mycoplasmas. FVU, less invasive self-collected specimen, can serve marker tract reliable way detecting carriers infection.