[18] Purification of stearyl-CoA desaturase from liver

摘要: Publisher Summary This chapter describes the purification of stearyl-CoA desaturase from liver. The isolation method described in includes minor modifications original procedure that can increase both reproducibility and yield preparations. liver rats induced for this enzyme utilizes successive detergent extractions microsomes to remove other proteins initially. principle rapid spectrophotometric assay is measurement rate reoxidation reduced cytochrome b 5 produced by addition a complete system presence oxygen limiting NADH. purified relatively labile, particularly detergents such as Triton X-100 or deoxycholate concentrations used preparation. Even at 0 °, fraction 8 loses 30-50% catalytic activity 15-20 hrs. accomplished iron loss protein.