Isolation and characterization of OLE1, a gene affecting fatty acid desaturation from Saccharomyces cerevisiae.

摘要: The unsaturated fatty acid (ufa) requiring ole1 mutant of Saccharomyces cerevisiae appears to produce a defective Δ-9 desaturase. This enzyme catalyzes double bond formation between carbons 9 and 10 palmitoyl stearoyl coenzyme A. A DNA fragment isolated by complementation an strain repairs the ufa requirement in cells. Genetic analysis cloned indicates that it is allelic OLE1 gene. Disruption single copy wild type gene diploid produces both nonreverting ufa-requiring haploid progeny upon sporulation. Membrane lipids disrupted strains contain only ufas supplied growth medium. recovery activity segregants was examined after removal from Following deprivation disruptant cells grew normally for about three generations then at slower rate least 0.6 generations. During time cellular dropped 63 7.3 mol % total acids. No production 16:1 18:1 products desaturase observed cells, whereas desaturation control evident 2 h deprivation. These results indicate 1) essential monounsaturated acids probably structural enzyme. 2) large part membrane present under normal culture conditions are not cell division.