Neurokinin-1 Receptor-Expressing Neurons That Contain Serotonin and Gamma-Aminobutyric Acid in the Rat Rostroventromedial Medulla Are Involved in Pain Processing
作者： Tao Chen , Xiao-Lin Wang , Juan Qu , Wei Wang , Ting Zhang
DOI: 10.1016/J.JPAIN.2013.02.002
关键词: Spinal cord 、 Neuroscience 、 Nucleus 、 Nucleus raphe magnus 、 Dorsal raphe nucleus 、 Periaqueductal gray 、 Substance P 、 Glutamate decarboxylase 、 Biology 、 gamma-Aminobutyric acid
摘要: Abstract The rostroventromedial medulla (RVM) is an important area of the endogenous pain-regulating system, in which 5-hydroxytryptamine (5-HT) and gamma-aminobutyric acid (GABA) are 2 main transmitters involved pain modulation. However, whether 5-HT GABA colocalized poorly understood. By using glutamate decarboxylase 67-green fluorescence protein (GAD67-GFP) knock-in mouse, we confirmed colocalization RVM, with a distribution raphe magnus nucleus paragigantocellular reticular nucleus. Interestingly, more than half (51.6%) 5-HT/GABA-immunoreactive (ir) neurons expressed neurokinin-1 receptors (NK-1R) one-third (30.1%) 5-HT/GABA/NK-1R-ir projected to spinal cord, suggesting that substance P (SP) should regulate activity 5-HT/GABA-ir cord-projecting neurons. combining retrograde anterograde tracing methods, observed cuneiform nucleus, dorsal lateral periaqueductal gray origin nuclei for SP-ir fibers terminals RVM. Finally, after formalin injection into mice hind paw, 29.2% RVM-projecting from supra-RVM 33.1% NK-1R-ir RVM were activated. present study provides potent morphological evidence coexistent RVM-spinal also regulated by SPergic projections. Perspective results will greatly enhance our understanding modulation nociceptive information descending system.
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