Methods to assay for post-transcriptional suppression of gene expression
作者： Paul W. Oeller , Neal Gutterson , John R. Bedbrook
DOI:
关键词: Reporter gene 、 Genetics 、 Biology 、 Gene silencing 、 Gene expression 、 Gene 、 Plant cell
摘要: This invention provides methods for identifying plant cells that exhibit post-transcriptional gene silencing (PTGS) of a chosen gene. The involve the use suppression-sensitive reporter genes that, when introduced into cells, are expressed at lower level in PTGS than not silenced particular
lens.org 参考文章(12)
Wolfgang W. Schuch, Donald Grierson, Ian G. Bridges, Dicot plants containing an antisense polygalacturonase gene segment ,(1989)
Richard A. Jorgensen, Carolyn A. Napoli, Genetic engineering of novel plant phenotypes ,(1990)
Sue L. Loesch-Fries, Nancy P. Jarvis, Donald J. Merlo, Virus resistant plants having antisense RNA ,(1987)
Taline Elmayan, Herve Vaucheret, Expression of single copies of a strongly expressed 35S transgene can be silenced post-transcriptionally Plant Journal. ,vol. 9, pp. 787- 797 ,(1996) , 10.1046/J.1365-313X.1996.9060787.X
G. Vancanneyt, R. Schmidt, A. O'Connor-Sanchez, L. Willmitzer, M. Rocha-Sosa, Construction of an intron-containing marker gene : splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation Molecular Genetics and Genomics. ,vol. 220, pp. 245- 250 ,(1990) , 10.1007/BF00260489
J Goodwin, K Chapman, S Swaney, T D Parks, E A Wernsman, W G Dougherty, Genetic and biochemical dissection of transgenic RNA-mediated virus resistance. The Plant Cell. ,vol. 8, pp. 95- 105 ,(1996) , 10.1105/TPC.8.1.95
Elisabeth Mueller, Julie Gilbert, Guy Davenport, Gianinna Brigneti, David C. Baulcombe, Homology‐dependent resistance: transgenic virus resistance in plants related to homology‐dependent gene silencing Plant Journal. ,vol. 7, pp. 1001- 1013 ,(1995) , 10.1046/J.1365-313X.1995.07061001.X
Susan M Angell, David C Baulcombe, Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA The EMBO Journal. ,vol. 16, pp. 3675- 3684 ,(1997) , 10.1093/EMBOJ/16.12.3675
David C. Baulcombe, RNA as a target and an initiator of post-transcriptional gene silencing in trangenic plants Plant Molecular Biology. ,vol. 32, pp. 79- 88 ,(1996) , 10.1007/BF00039378
Karla Jean Fullner, Eugene W Nester, None, Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens. Journal of Bacteriology. ,vol. 178, pp. 1498- 1504 ,(1996) , 10.1128/JB.178.6.1498-1504.1996