Genetic and biochemical dissection of transgenic RNA-mediated virus resistance.

摘要: RNA-mediated virus resistance has been observed in transgenic plants at varying frequencies, suggesting that a nuclear requirement or other pre-condition must be met. This study was undertaken to characterize genetically transgenes confer highly resistant state infection by tobacco etch (TEV). Transgenic line 2RC-6.13, expressing an untranslatable mRNA containing the TEV coat protein open reading frame, had three distinct transgene integration events segregated as two linkage groups. A genetic series of contained zero, one, two, all inserts both homozygous and heterozygous conditions produced examined. Genetic biochemical data suggested is multigenic trait 2RC-6.13; more were necessary establish state. One copies resulted inducible form (i.e., recovery). Transcription rates steady RNA levels transgene-derived transcript present different members supported post-transcriptional degradation process underlying mechanism for reduction resistance. appeared initiate via cleavage specific sites within target sequence, determined get blot primer extension analyses from various plant lines.