Transduced Tat-Annexin protein suppresses inflammation-associated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells.
作者： Sun-Hwa Lee , Dae-Won Kim , Su-Sun Back , Hyun-Sook Hwang , Eun-Young Park
DOI: 10.5483/BMBREP.2011.44.7.484
关键词: Annexin 、 Cytokine 、 MAPK/ERK pathway 、 NFKB1 、 Molecular biology 、 Prostaglandin E 、 Chemistry 、 Inflammation 、 Protein kinase A 、 RAW 264.7 Cells
摘要: Annexin-1 (ANX1) is an anti-inflammatory protein as well important modulator in inflammation. However, the precise action of ANX1 remains unclear. To elucidate protective effects on lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells, we constructed a cell-permeable Tat-ANX1 protein. The transduced markedly inhibited expression cyclooxygenase-2, production prostaglandin E(2), and generation pro-inflammatory cytokines cells. Furthermore, caused significant reduction activation nuclear factor- kappa B (NF-kB) mitogen-activated kinase (MAPK). results indicate that inhibits inflammatory response enzymes by blocking NF-kB MAPK. Therefore, may be useful therapeutic agent against various diseases.
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