Genetic analysis and phenotypic associations for drought tolerance in Hordeum spontaneum introgression lines using SSR and SNP markers

摘要: Associations between markers and drought related traits were investigated on a set of 57 advanced barley breeding lines, carrying various levels introgression from Hordeum spontaneum lines 41-1 41-5, the best sources tolerance in ICARDA program, using 74 simple sequences repeats (SSR) 20 single nucleotide polymorphism markers. The evaluated for grain yield three years (2003/04, 2004/05, 2005/06) nine Mediterranean low rainfall environments. A high level was found with SSR markers, mean information content gene diversity values 0.67 0.71, respectively. number alleles per locus varied 2 to 11, an average 5.8 marker. Considering all linkage disequilibrium (LD) analysis significant at comparison-wise P < 0.01 nearly 9 % marker pairs used decay LD observed value r2 0.2 genetic distance 40 cM. association revealed total 147 marker–trait associations traits. 72 (49 %) showed favorable effects exotic germplasm where H. contributed improvement trait under stress conditions. were: 12 growth habit; vigor; 11 peduncle extrusion; 5 grains spike; length; 16 days heading; plant height; 8 spike 17 thousand kernel weight; 30 yield; 4 harvest index biological yield. phenotypic variation explained by individual ranged 7.6 36.2 %. identification genomic regions associated is useful cultivars better adapted Thus, present study encouraging identifying through based mapping analysis, which could complement augment previous quantitative loci potential use Marker Assisted Selection drought.