Analysis of simple sequence repeats (SSRs) in wild barley from the Fertile Crescent: associations with ecology, geography and flowering time.

作者: Victor Ivandic , Christine A. Hackett , Eviatar Nevo , Richard Keith , William T.B. Thomas

DOI: 10.1023/A:1014875800036

关键词: BotanyAnalysis of molecular varianceHordeum vulgareBiologyGenetic diversityMicrosatelliteEcologyGenotypeAlleleGenetic variationHordeum

摘要: Wild barley, Hordeum spontaneum C. Koch, is the progenitor of cultivated vulgare. The centre diversity in Fertile Crescent Near East, where wild barley grows a wide range conditions (temperature, water availability, day length, etc.). genetic 39 genotypes collected from Israel, Turkey and Iran was studied with 33 SSRs known map location. Analysis molecular variance (AMOVA) performed to partition variation present within between three countries origin. Using classification tree analysis, two (or three) specific were identified which could correctly classify most according country Associations SSR flowering time adaptation site-of-origin ecology geography investigated by contrasting statistical approaches, linear regression based on length allele class differences. A number significantly associated under four different growing regimes (short days, long unvernalised vernalised). Most associations observed be accounted for close linkage loci earliness per se genes. No found photoperiodic vernalisation response genes control suggesting that factors may active barley. Novel genomic regions controlling detected chromosomes 1HS, 2HL, 3HS 4HS. ecological geographic data primarily determining plant development. This study shows analyses repeat are complementary, some not necessarily selectively neutral.

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