Nuclear localization of the major vault protein in U373 cells.
作者： Marco Slesina , Elisabeth M. Inman , Leonard H. Rome , Walter Volknandt
DOI: 10.1007/S00441-005-1086-8
关键词: Nuclear protein 、 Nuclear pore 、 Nucleus 、 Cell biology 、 Nucleoplasm 、 Biology 、 Nuclear localization sequence 、 Vault (organelle) 、 Major vault protein 、 Astroglioma
摘要: The major vault protein (MVP) is the predominant member of a large ribonucleoprotein particle, named vault. Vaults are abundant in cytosol mammalian cells. Mammalian MVP has previously been reported to be associated with nucleus, particularly its cytosolic surface on which vaults thought dock at or near nuclear pore complex. To date presence particles inside nucleus convincingly only for sea urchin We have addressed potential localization cells by employing confocal laser microscopy and cryo-immunoelectron microscopy. As revealed immunostaining analysis transfected construct encoding green fluorescent protein, present both nucleus. Cryo-electron human astroglioma U373 reveals clusters immunogold pores nucleoplasm suggesting that particulate structures. Quantification fluorescence observed nuclei about 5% localized Our results further support notion part cellular can enter
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