Arrays having background features and methods for using the same

作者: Theodore R. Sana , Glenda C. Delenstarr , Paul K. Wolber

DOI:

关键词: Artificial intelligenceSignalAnalytical chemistryNucleic acidAnalyteFeature (computer vision)Pattern recognitionBiology

摘要: Nucleic acid arrays that have background features, and methods for using the same, are provided. The subject nucleic include both hybridization features where provide a signal in assay is made up of feature substrate component, probe component non-specific binding component. In practicing methods, contacted with sample signals observed features. then subtracted from to obtain corrected employed as output assay, e.g., determine presence, either qualitatively or quantitatively, analyte target sample. Also provided kits use methods.

参考文章(53)
House Fs, Davidson Jm, Henderson Gs, Stewart Sj, Conary Jt, McCurley Tl, A reliable method for northern blot analysis using synthetic oligonucleotide probes. BioTechniques. ,vol. 10, pp. 190- 197 ,(1991)
Christine Debouck, Derk Bergsma, Martin Rosenberg, Differentially expressed genes in healthy and diseased subjects ,(1995)
Mark J. Fiandaca, Jens J. Hyldig-Nielsen, Kyriaki Stefano, James M. Coull, Sven E. Godtfredsen, Methods, kits and compositions for suppressing the binding of detectable probes to non-target sequences in hybridization assays ,(1997)
Mickey Urdea, Brian Warner, Solution phase nucleic acid sandwich assay ,(1985)
R. J. Lipshutz, D. Morris, M. Chee, E. Hubbell, M. J. Kozal, N. Shah, N. Shen, R. Yang, S. P. A. Fodor, Using oligonucleotide probe arrays to access genetic diversity Microsystem Technology: A Powerful Tool for Biomolecular Studies. ,vol. 19, pp. 241- 254 ,(1999) , 10.1007/978-3-0348-8817-2_9
Richard H. Scheuermann, Steven R. Bauer, Polymerase chain reaction-based mRNA quantification using an internal standard: analysis of oncogene expression. Methods in Enzymology. ,vol. 218, pp. 446- 473 ,(1993) , 10.1016/0076-6879(93)18035-B
A Ravaggi, A Zonaro, C Mazza, A Albertini, E Cariani, Quantification of hepatitis C virus RNA by competitive amplification of RNA from denatured serum and hybridization on microtiter plates. Journal of Clinical Microbiology. ,vol. 33, pp. 265- 269 ,(1995) , 10.1128/JCM.33.2.265-269.1995