Methods and reagents for cleaving and deprotecting oligonucleotides
作者： Parameswara Meda Reddy , Naeem Botros Hanna
DOI:
关键词: Organic chemistry 、 Oligonucleotide 、 Methylamine 、 Chemistry 、 Reagent
摘要: Disclosed are reagents of methods for cleaving and deprotecting insolubilized protected synthetic oligonucleotides. In a particularly preferred embodiment, the reagent comprises methylamine t-butylamine.
google.com.br 参考文章(23)
Robert L. Letsinger, Method of deprotecting polynucleotide trimethylethyl phosphotriesters ,(1984)
Hansen Maxwell Hsiung, Process for de-cyanoethylating blocked nucleotides ,(1981)
H. Köster, J. Biernat, J. McManus, A. Wolter, A. Stumpe, Ch.K. Narang, N.D. Sinha, Polymer support oligonucleotide synthesis-XV: Synthesis of oligodeoxynucleotides on controlled pore glass (CPG) using phosphate and a new phosphite triester approach☆☆☆ Tetrahedron. ,vol. 40, pp. 103- 112 ,(1984) , 10.1016/0040-4020(84)85108-X
Edward L. Johnson, Thomas O. Sidebottom, William E. Rich, Method and apparatus for quantitative analysis of weakly ionized anions or cations ,(1979)
Hubert Köster, Konrad Kulikowski, Thomas Liese, Wiebke Heikens, Vipin Kohli, N-acyl protecting groups for deoxynucleosides : A quantitative and comparative study Tetrahedron. ,vol. 37, pp. 363- 369 ,(1981) , 10.1016/S0040-4020(01)92022-8
Steven Fung, Steven M. Menchen, Sam L. Woo, Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports ,(1987)
Didier Molko, Jean-Claude Schulhof, Robert Teoule, Protected nucleosides which permit more efficient oligonucleotide syntheses ,(1987)
L.J. McBride, M.H. Caruthers, An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides Tetrahedron Letters. ,vol. 24, pp. 245- 248 ,(1983) , 10.1016/S0040-4039(00)81376-3
R. K. SINGH, A. DIKSHIT, M. CHADDHA, G. WATAL, K. MISRA, Protecting Groups Used in Oligonucleotide Synthesis: A Current Survey ChemInform. ,vol. 22, ,(1991) , 10.1002/CHIN.199114321
Huynh Vu, Christie McCollum, Karen Jacobson, Pete Theisen, Ravi Vinayak, Elizabeth Spiess, Alex Andrus, Fast oligonucleotide deprotection phosphoramidite chemistry for DNA synthesis Tetrahedron Letters. ,vol. 31, pp. 7269- 7272 ,(1990) , 10.1016/S0040-4039(00)88541-X