Reagents for oligonucleotide cleavage and deprotection
作者： Jeffrey S. Nelson
DOI:
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摘要: The present invention provides a process for the removal of protecting groups, i.e. deprotection, from chemically synthesized oligonucleotides. In one embodiment, reagents suitable use in such process, and kits incorporating convenient, ready-to-use format. By invention, side-reactions leading to certain impurities that contaminate oligonucleotides can be minimized. Methods are provided deprotection an oligonucleotide by reacting protected with reagent wherein comprises active methylene compound amine reagent. has structure (a) where substituent EWG is electron-withdrawing group R hydrogen, C1-C12 alkyl, C6-C20 aryl, heterocycle or group.
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