Process for separating and deprotecting oligonucleotides
作者： Marek Kwiatkowski
DOI:
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摘要: A method for deprotecting oligonucleotides comprising: (a) providing protected wherein (i) the 5' end is linked to a hydrophobic separation function (e.g. dimethoxytrityl) or (ii) both ends are pair of functions in which one member less than other being alkyl, aryalkyl arylsiloxyl); (b) precipitating by drying) on support polystyrene-based support) using an organic solvent, thereby non-covalently immobilizing and making them insoluble; (c) (i.e. removing functions) reagent solvent 2 % trichloroacetic acid dichloromethane).
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