N-6-Dialkylformamidine-2′-deoxyadenosine Phosphoramidites in Oligodeoxynucleotide Synthesis Raped Deprotection of Oligodeoxynucleotides

作者: Pete Theisen , Christie McCollumy , Alex Andrus

DOI: 10.1080/07328319308016202

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摘要: Abstract A series of dialkylformamidine protected deoxyadenosine phosphoramidites were prepared for automated, solid-support DNA synthesis. The set Abz, Gdmf, Cbz, T gave high purity and yield oligodeoxynucleotides, with complete deprotection at 65 °C in one hour. Different times temperatures exposure to concentrated ammonium hydroxide examined establish the optimum conditions oligodeoxynucleotides.

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