Process for the manufacture of oligonucleotides
作者： Hagen Cramer , Kevin James Finn , Tadeusz Krzysztof Wyrzykiewicz , Huihe Zhu
DOI:
关键词: Oligonucleotide 、 Combinatorial chemistry 、 Conjugate acid 、 Solvent 、 Pyridine 、 Amine gas treating 、 Organic chemistry 、 Chemistry
摘要: A process for manufacturing an oligonucleotide which comprises removing β-eliminating phosphorus-protecting groups, in particular β-cyanoethyl protective groups from a protected oligonucleotide, wherein said contacting the with amine solution solvent preferably does not consist of pyridine, conjugate acid has pKa greater than 11.5, and concentration is less 0.5 mole/liters.
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