作者: P. Kaspar , G. Moeller , A. W. Wahlefeld , F. Staehler
DOI: 10.1007/BF00481761
关键词: Centrifugation 、 Solubilization 、 Suspended particles 、 Chymotrypsin 、 Particle 、 Chemistry 、 Reactivity (chemistry) 、 Small sample 、 Absorption (chemistry) 、 Chromatography
摘要: The proposed method offers the following advantages: a) high reactivity of enzyme; b) need for small sample volume, thus absorption from suspended particles does not interfere and c) nearly complete solubilization particle bound enzyme allowing automated analysis with supernatant after centrifugation.